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Making Mushroom Spore Prints: A SporesWorldwide Guide

Learning how to make a mushroom spore print is an essential skill for any aspiring mycologist. Whether you’re an avid mushroom forager or interested in cultivating your own mushrooms, spore printing is a valuable technique for mushroom identification and completing the mushroom life cycle. In this comprehensive guide, we will explore the process of making mushroom spore prints, its various applications, and provide step-by-step instructions to help you master this technique.

What are Spores?

Mushroom spores, often referred to as “seeds,” carry half of the genetic information required to produce a mushroom. These microscopic structures are released from the gills or pores located under the mushroom cap. Dispersed by air currents, spores can land in suitable environments and develop into mycelium, which eventually forms a new mushroom. While spores come in different shapes, colors, and sizes, the most visible characteristic to the human eye is the spore color, making spore prints crucial for accurate mushroom identification.

How to Make a Mushroom Spore Print

The process of making a spore print is relatively straightforward and can be applied to both gilled mushrooms and those with pores. Here’s a step-by-step guide to help you create your own spore prints:

Step 1: Choose a Mushroom

Select a mature mushroom that has reached the spore-producing stage. Avoid picking mushrooms that are too young, as they may not have released enough spores for a successful print. Additionally, ensure the mushroom’s gills or pores are exposed for spore deposition.

Step 2: Prepare the Mushroom Cap

Carefully detach the mushroom cap from the stem, preferably at the highest point possible. For certain varieties like shelf mushrooms or oyster mushrooms, using the entire fruiting body may be suitable for creating the spore print.

Step 3: Position the Cap for Printing

Place the mushroom cap with the gills or pores facing downwards onto a surface suitable for spore deposition. For most mushrooms, a regular sheet of white paper works well. However, if you require precise identification, consider using black paper for better contrast. If you plan to grow mushrooms from spores, tinfoil is recommended due to its sterility and ease of transferring spores.

Step 4: Cover and Wait

Cover the mushroom cap with a glass or small bowl to prevent air currents from dispersing the spores. This enclosure helps ensure a clear and uncontaminated spore print. Allow the mushroom to sit undisturbed for 6 to 12 hours to allow ample time for spore release and deposition.

Step 5: Retrieve the Spore Print

After the designated time, carefully remove the glass or bowl covering the mushroom cap. You should find a fully formed spore print on the surface below. To preserve the print, fold the paper or tinfoil, enclosing the spores, and store it in a ziplock bag. Spore prints can be kept at room temperature for an extended period, remaining viable for years without the need for refrigeration.

Mushroom Spore Prints for Identification

Spore prints are invaluable for identifying different mushroom species, primarily based on their spore color. However, it’s essential to exercise caution and not rely solely on spore color for mushroom edibility determination. Variation in color perception among individuals can make precise identification challenging. Therefore, it’s advisable to use additional identification methods and create multiple spore prints on different colored papers when unsure.

Spore Prints for Mushroom Cultivation

While growing mushrooms from spores can be unpredictable due to genetic variation, making spore prints is an excellent technique for capturing new genetics and expanding your mushroom cultivation repertoire. Here’s an overview of the process:

  1. Germinating Spores on Agar Plates: a. Sterilize a scalpel and touch the spore print to collect numerous spores. b. Streak the scalpel tip across the surface of a nutrified agar plate, creating an “S” pattern. c. After 5 to 15 days, spores should germinate, forming distinct colonies that can be transferred to additional agar plates for cultivation.
  2. Creating a Spore Syringe: a. Sterilize a syringe and fill it with sterile water. b. Collect a small amount of spores from the spore print and inject them into the syringe. c. The spore syringe can be used to inoculate various mushroom substrates, increasing the chances of successful germination and cultivation.


Spore printing is a simple yet fascinating technique that allows mushroom enthusiasts to explore the world of fungi in multiple ways. From identifying mushroom species to expanding genetic diversity in mushroom cultivation, spore prints offer a wealth of opportunities. Remember to handle the process with care, ensuring cleanliness and sterile conditions to maximize the success of your spore prints. With this comprehensive guide, you’re now equipped to embark on your own mushroom spore printing adventures and uncover the hidden wonders of the fungal kingdom.

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